Microbiology Techniques

Biological Indicator For Heat Sterilisation

1 PARAMETERS OF BIOLOGICAL INDICATORS FOR HEAT STERILISATION 1-1 z-Value Sterilisation processes can be operated at temperatures lower than the standard 121 °C (for longer exposure times) or at higher temperatures (for shorter exposure times). The z-value (the temperature difference that leads to a 10-fold change in the D-value of the biological indicator) is used to compare the efficacy …

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SOP For Microbial Culture Management

A microbial culture (microbiological culture) is a procedure of growing microbial organisms (reproduction) by allowing them to breed in programmed culture medium under controlled laboratory conditions. Microbial cultures are initial and basic diagnostic methods used as a research tool in molecular biology. Microbial Culture Management Standard Operating Procedure (SOP) PURPOSE: To lay down the procedure …

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SOP for Media Fill Validation

Standard Operating Procedure (SOP) for Media Fill Validation in Sterile facility.  A “media fill” (sometimes known as a “process simulation”) is the performance of an aseptic manufacturing procedure using a sterile microbiological growth medium in place of the drug solution. The microbiological growth medium is used in place of the drug solution during media fills …

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SOP on Maintenance and Preparation of Anaerobic Culture Suspension.

SOP on Maintenance and Preparation of Anaerobic Culture Suspension ( Clostridium Sporogenes ). This procedure is for Maintenance & preparation of culture suspension for anaerobic microorganisms in Microbiology Department.  it includes maintenance of culture & preparation of culture suspension as per Culture Dilution Method. PROCEDURE: Maintenance: Prepare Nutrient Agar as per Media preparation SOP Pour 5 mL …

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